I really love this anime and you guys should check this out. one of the most brutal animes I have came across. The series is set in Japan during the mid-Tokugawa Shogunate period, beginning in the 2nd year of the Tenmei era or 1782.
blade of the Immortal follows the deeds of Manji, a skilled samurai
who has a decisive advantage: no wound can kill him, except for a rare
poison. In the past, his criminal actions led to the death of 100 other
samurai (including his sister's husband). He becomes immortal at the
hand of an 800-year-old nun named Yaobikuni,
and is compelled by the death of his sister to accept the quest that
will end his agelessness. He has vowed to make amends by killing 1000
evil men, and until he does Manji will be kept alive by "sacred
bloodworms". He swore that he will kill 1000 people in order to reverse the immortality.